TY  - BOOK
AU  - Muyzer,G.
AU  - De Waal,E.C.
AU  - Uitierlinden,A.G.
TI  - Profiling of Complex Microbial Populations by Denaturing Gradient Gel Electrophoresis Analysis of Polymerase Chain Reaction-Amplified Genes Coding for 16S rRNA
N2  - We describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. This technique is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16S rRNA, all the same length, by denaturing gradient gel electrophoresis (DGGE). DGGE analysis of different microbial communities demonstrated the presence of up to 10 distinguishable bands in the separation pattern, which were most likely derived from as many different species constituting these populations, and thereby generated a DGGE profile of the populations. We showed that it is possible to identify constituents which represent only 1
UR  - https://drive.google.com/file/d/1taQYuXl7XqQlbFSpRGLMj2AXhn6eHxdZ/view?usp=drivesdk
ER  -