TY  - BOOK
AU  - Gong,Z.Y.
AU  - He,Z.S.
AU  - Zhu,J.B.
AU  - Yu,G.Q.
AU  - ZouH.S.
TI  - Sinorhizobium meliloti nifA mutant induces different gene expression profile from wild type in Alfalfa nodules
KW  - CDNA-AFLP
KW  - GENE EXPRESSION
KW  - NODULE
KW  - SINORHIZOBIUM MELILOTI
KW  - NIFA
N2  - Several studies have demonstrated that the Rhizobium nifA gene is an activator of nitrogen fixation acting in nodule bacteria. To understand the effects of the Sinorhizobium meliloti nifA gene on Alfalfa, the cDNA-AFLP technique was employed to study the changes in gene expression in nifA mutant nodules. Among the approximately 3,000 transcriptderived fragments, 37 had differential expression levels. These expression levels were subsequently confirmed by reverse Northern blot and RT-polymerase chain reaction. Sequence analyses revealed that 21 cDNA fragments corresponded to genes involved in signal communication, protein degradation, nutrient metabolism, cell growth and development
UR  - https://drive.google.com/file/d/1m268xDN51th-qcmemiMUUexCy4tQLm9o/view?usp=drivesdk
ER  -