Molecular and biochemical evidence for manganese-dependent peroxidase activity in Tylospora 

 -  Mycological Research, 103(9), p.1098-1102, 1999 .
<br/><br/> Gel-based and spectrophotometric assays were used to demonstrate expression of a manganese-dependent peroxidase (MnP)activity in culture ®ltrates of the ectomycorrhizal basidiomycete Tylospora ®brillosa. PCR ampli®cation using a primer pair speci®c for a 260 bp fragment from the 5«-end of a gene encoding an H3-like MnP isozyme in Phanerochaete chrysosporium, produced single ampli®cation products of 260 bp from DNA extracted from two isolates of T. ®brillosa. The ampli®ed fragment from T. ®brillosa had a 93±7