TY  - BOOK
AU  - Chaturvedi,C.P.
AU  - Lodhi,N.
AU  - Ansari,S.A.
AU  - Tiwari,S.
AU  - Srivastava,R.
AU  - Sawant,S.V.
AU  - Tuli,R.
TI  - Mutated TATA-box/TATA binding protein complementation system for regulated transgene expression in tobacco
KW  - LEGUMIN PROMOTER
KW  - TATA BOX
KW  - TATA BINDING PROTEIN
KW  - TBP MUTANT
KW  - EXPRESSION MODULE
KW  - TIGHTLY REGULATED PROMOTER
N2  - A two-component expression system was developed to achieve tightly regulated expression of transgenes in plants. One component functioned as an expression module whereas the other functioned as a regulatory module. The expression module comprised a highly expressing TATA-dependent seed-specific promoter in which the TATA motif in the core promoter was mutated to TGTA. The regulatory module expressed a mutated general transcription factor TBPm3 that recognized TGTA and initiated transcription. Vectors were designed using component one alone or in combination with component two, and were transformed into tobacco. The TGTA mutation in the TATA-box completely inactivated the promoter, making component one non-functional. This non-functional module became transcriptionally active in the presence of the component two that expressed TBPm3. The reporter gene gusA was expressed from the TGTA-containing chimeric legumin promoter, in a tightly seed-specific manner, in transgenic tobacco plants in the presence of TBPm3 that was expressed from a constitutive promoter. The results show that the TGTA and TBPm3 combination can be used to achieve high-level tissue-specific expression of TATA-dependent promoters
UR  - https://drive.google.com/file/d/1a0HXXdn15XH2jtY0X_uYTfG9fiP3EIuh/view?usp=drivesdk
ER  -