TY  - BOOK
AU  - Laurinavichene,T.V.
AU  - Tolstygina,I.V.
AU  - Galiulina,R.R.
AU  - Ghirardi,M.L.
AU  - Seibert,M.
AU  - Tsygankov,A.A.
TI  - Dilution methods to deprive Chlamydomonas reinhardtii cultures of sulfur for subsequent hydrogen photoproduction
KW  - PHOTOBIOLOGICAL HYDROGEN PRODUCTION
KW  - SULFUR DEPRIVATION
KW  - GREEN ALGAE
KW  - PHOTOBIOREACTORS
N2  - Sulfur deprivation of Chlamydomonas reinhardtii cultures gradually inactivates photosynthetic O2 evolution and leads to the establishment of anaerobiosis in the medium. Sulfur-deprived algal cultures kept under anaerobic conditions will then produce H2 gas for 3-5 days under continuous illumination. Currently, sulfur deprivation is achieved by mechanical centrifugation of cultures grown in sulfur-replete medium, followed by extensive and costly washing. The cells are 8nally resuspended in sulfur-free medium. The current study investigates two procedures to deprive algal cultures of sulfur that eliminate the centrifugation step. These procedures involve sulfur deprivation by dilution of sulfur-replete cultures into either sulfur-limited medium or sulfur-free medium. We demonstrate that e:cient H2 photoproduction can be achieved on a timely basis using either procedure. However, the dilution of sulfate-replete algal cultures 1:10 v=v into sulfur-free medium is the most appropriate procedure. These observations serve as the basis for developing an algal H2-production system that is cheaper, less time-consuming, and less amenable to contamination with other microorganisms than systems employing centrifugation for sulfur deprivation
UR  - https://drive.google.com/file/d/1mwEtyxZQJS4GKBrN6UgEJi_FSQp_DpA7/view?usp=drivesdk
ER  -