TY  - BOOK
AU  - Jen,J.F.
AU  - Zen,J.H.
AU  - Cheng,F.C.
AU  - Yang,G.Y.
TI  - Immobilization of glucosidase onto silica-based, amino functionalized beads for enzymatic hydrolysis of urinary phenol prior to liquid chromatographic analysis
KW  - ENZYMATIC METHODS
KW  - LIQUID CHROMATOGRAPHY
KW  - PACKING MATERIALS
KW  - GLUCOSIDASE IMMOBILIZATION
KW  - PHENOL
KW  - URINARY PHENOL
N2  - An amino-functionalized silica commercial liquid chromatography packing material was used instead of controlled- pore glass as the support to react with glutaraldehyde and then to directly immobilize the /3-glucosidase. After enzymatic hydrolysis, the urinary phenol was separated by reversed-phase liquid chromatography and monitored by an electrochemical detector at 0.85 V (vs. Ag/AgCl). Factors that affect the immobilization and hydrolysis were investigated. The proposed method provides a simpler procedure with a less expensive material for glucosidase immobilization, and the electrochemical detection avoids the interference from other urinary species and hence simplifies the chromatogram obtained. The detection limit was 0.2 ng with a 28 ~1 injection. The relative standard deviations for 1.2 ng and 2.0 ng levels of phenols were 1.16
UR  - https://drive.google.com/file/d/1ItNie1pJVIj6OGN3WdRX0UiARS5_S5iE/view?usp=drivesdk
ER  -