Selective Solvent Extraction of Ruberythric Acid from Madder Roots and Subsequent Hydrolysis with Beta-Glucosidase 

 -  Journal of Fermentation and BioEngineering, 8186), p.567-569, 1996 .
<br/><br/> Selective solvent extraction of anthraquinones (alizarin and lucidin)and their prlmeverosides (ruherythric acid and lucidin-3-o-primeveroside)from madder roots could be achieved using two-phase extractm with chloroform (CHCl& and potassium hydroxide aqueous solution (KOH,). Ruberythrlc acid extracted to the aqueous phase could be selectively and completely converted to aliznrln within 360 mln by enzymatic hydrolysis with B-glucosidase at 50°C and at pH 5, reducing the inhibitory effect on the reaction caused by alizarin, which is not extracted in appreciable amounts into the aqueous phase, without appreciable hydrolysis of ldcidln-3- o-primeveroside. It was also found that ruberythrlc acid extracted from madder hairy roots by CHC13- KOH,, two-phase extraction was efficiently converted to alizarin in the same manner. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>SOLVENT EXTRACTION<br/>RUBERYTHRIC ACID<br/>MADDER ROOT<br/>ENZYMATIC HYDROLYSIS