TY  - BOOK
AU  - Ge,Q.
AU  - Liu,Z.
AU  - Bai,Y.
AU  - Zhang,D.
AU  - Yu,P.
AU  - Lu,Z.
TI  - Emulsion PCR-based method to detect Y chromosome microdeletions
KW  - EMULSIONS
KW  - MULTIPLEX PCR
KW  - Y CHROMOSOME MICRODELETIONS
N2  - Deletions in Y chromosome are thought to be pathologically involved in some cases of male infertility associated with azoospermia or oligozoospermia. An emulsion-based multiplex PCR method was developed for detecting Y chromosome microdeletions in infertile men and a plasma sample of pregnant women carrying a male fetus. The sensitivity of multiplex PCR in emulsion was evaluated. Conventional PCR was also carried out for comparison. A total of 13 sequence-tagged sites (STSs)distributed in the AZF region were analyzed simultaneously with this method. The SRY gene was also detected as the inner control. Results showed that Y chromosome microdeletions were found in 4 of 19 infertile patients. Also, in 1 of 63 samples collected from pregnant women, microdeletions were found in some of the detected sites. It is suggested that the emulsion PCR assay was proven to be a promising diagnostic tool and could be widely used in further clinical and academic research
UR  - https://drive.google.com/file/d/1lRYB7RBZhI5cKmK3S5wX4zmf1f2llgJu/view?usp=drivesdk
ER  -