TY  - BOOK
AU  - Debrabant,A.
AU  - Joshi,M.B.
AU  - Pimenta,P.F.P.
AU  - Dwyer,D.M.
TI  - Generation of Leishmania donovani axenic amastigotes: their growth and biological characteristics
KW  - TRYPANOSOMATID
KW  - LEISHMANIASIS
KW  - PARASITE
KW  - CULTURE SYSTEM
KW  - INFECTIVITY
KW  - VIRULENCE
N2  - In this report, we describe an in vitro culture system for the generation and propagation of axenic amastigotes from the well characterised 1S-CL2D line of Leishmania donovani. Fine structure analyses of these in vitro-grown amastigotes demonstrated that they possessed morphological features characteristic of L. donovani tissue-derived amastigotes. Further, these axenic amastigotes (LdAxAm)were shown to synthesise and release a secretory acid phosphatase isoform similar to that produced by intracellular amastigotes. Such LdAxAm also expressed surface membrane 30-nucleotidase enzyme activity similar to that of tissue-derived amastigotes. Moreover, LdAxAm, in contrast to promastigotes, expressed significant levels of the amastigote-specific A2 proteins. In addition, LdAxAm, derived from long term cultures of Ld 1S-CL2D promastigotes, had significant infectivity for both human macrophages in vitro and for hamsters in vivo. Thus, the in vitro culture system described herein provides a useful tool for the generation of large quantities of uniform populations of axenic amastigotes of the L. donovani 1S-CL2D line. The availability of such material should greatly facilitate studies concerning the cell and molecular biology of this parasite developmental stage
UR  - https://drive.google.com/file/d/18cfTZqP-Hd9DOWpytgkMPaYfad5mK5l0/view?usp=drivesdk
ER  -