Aluminum-tolerant Protoplasts from Roots Can be Collected with Positively Charged Silica Microbeads: a Method Based on Differences in Surface Negativity - Plant & Cell Physiology, 36(8), p.1493-1502, 1995 .

A new technique was developed for the collection of Al-tolerant plant cells. The procedure takes advantage of the difference in surface charge between Al-tolerant and Al-sensitive protoplasts. Equal volumes of freshly prepared, positively charged silica microbeads (0.05


ALUMINUM (AL)TOLERANCE
POSITIVELY CHARGED SILICA MICROBEADS
PROTOPLAST
SURFACE NEGATIVITY.