TY - BOOK AU - Wagatsuma,T. AU - Jujo,K. AU - Ishikawa,S. AU - Nakashima,T. TI - Aluminum-tolerant Protoplasts from Roots Can be Collected with Positively Charged Silica Microbeads: a Method Based on Differences in Surface Negativity KW - ALUMINUM (AL)TOLERANCE KW - POSITIVELY CHARGED SILICA MICROBEADS KW - PROTOPLAST KW - SURFACE NEGATIVITY N2 - A new technique was developed for the collection of Al-tolerant plant cells. The procedure takes advantage of the difference in surface charge between Al-tolerant and Al-sensitive protoplasts. Equal volumes of freshly prepared, positively charged silica microbeads (0.05 UR - https://drive.google.com/file/d/1y2ufjs-TrDjvBrDX3IEoLXlak3Acr4DB/view?usp=drivesdk ER -