TY  - BOOK
AU  - Wagatsuma,T.
AU  - Hossain Khan,M.S.
AU  - Rao,I.M.
AU  - Wenzl,P.
AU  - Tawaraya,K.
AU  - Yamamoto,T.
AU  - Kawamura,T.
AU  - Kawamura,T.
AU  - Ishikawa,S.
TI  - Methylene Blue Stainability of Root-lSp Protoplasts as an Indicator of Aluminum Tolerance in a Wide Range of Plant Species, Cultivars and Lines
KW  - ALUMINUM TOLERANCE
KW  - METHYLENE BLUE STAINABILITY
KW  - PLASMA MEMBRANE NEGATIVITY
KW  - PLASMA MEMBRANE PERMEABILITY
KW  - PROTOPLAST
N2  - We previously developed a new simple technique of methylene blue (MB)staining for the discrimination of aluminum (Al)-tolerant protoplasts from 4 plant species (rice, oats, maize and pea). The objectives of the present study were to conflrm the applicability of this technique to a wider range of plant species, cultivars and lines, and to identify a common strategy for the early stage of Al tolerance. A total of 10 plant species, two rachiaria spp., two Oryza spp., buckwheat, maize, pea, triticale, wheat and barley, corresponding to 18 different plant samples (species, cultivars, and lines), were used. Al tolerance (relative net root elongation of the longest root), which was screened at 20 pM AlC13 in 0.2 mM CaC12 (pH 4.9)for 24 h, ranged widely from 10 to 88. Among cultivars and lines within the same species, Al accumulation in the root tip portion was higher in Al-sensitive plants, corresponding to more severe permeabilization of the plasma membranes (PM). Protoplasts isolated mainly from the epidermis, and outer and central parts of the cortex were stained to different degrees by MB, and the blue color was observed both on the surface and inside the protopladlts. Color pictures obtained after staining for 3 min with 0.1
UR  - https://drive.google.com/file/d/1oG4E5suwCM-7vcrC6rk9xsYbvL8xY1tr/view?usp=drivesdk
ER  -