TY  - BOOK
AU  - Welda,R.J.
AU  - Colin,C.E.
AU  - Ridgwaya,H.J.
TI  - Agrobacterium-mediated transformation of Sclerotinia sclerotiorum
N2  - Ascospores from the phytopathogenic fungus Sclerotinia sclerotiorum were transformed to hygromycin B resistance by co-cultivation with Agrobacterium tumefaciens. Transformed spores germinated and grew on PDA supplemented with 100 ug/ml hygromycin B. The presence of mitotically stable hph gene integration at random sites in the genome was confirmed by PCR and Southern blot analysis. A transformation frequency of 8 × 10- 5 was achieved in five separate experiments. This study is the first report of success co-cultivating A. tumefaciens with S. sclerotiorum. This report of a reproducible Agrobacterium-mediated transformation method should allow the development of T-DNA tagging as a system for insertional mutagenesis in S. sclerotiorum and provide a simple and reliable method for genetic manipulation
UR  - https://drive.google.com/file/d/1lMPEK8erbud-SSE3qm_hK2_EguHy1yDp/view?usp=drivesdk
ER  -