Papaya shoot tip associated endophytic bacteria isolated from in vitro cultures and host-endophyte interaction in vitro and in vivo
- Canadian Journal of MicroBiology, 53(3), p.380-390, 2007 .
Fourteen distinct bacterial clones were isolated from surface-sterilized shoot tips (~1 cm)of papaya (Carica papaya L. 'Surya')planted on Murashige and Skoog (MS)-based papaya culture medium (23/50 nos.)during the 2-4 week period following in vitro culturing. These isolates were ascribed to six Gram-negative genera, namely Pantoea (P. ananatis), Enterobacter (E. cloacae), Brevundimonas (B. aurantiaca), Sphingomonas, Methylobacterium (M. rhodesianum), and Agrobacterium (A. tumefaciens)or two Gram-positive genera, Microbacterium (M. esteraromaticum)and Bacillus (B. benzoevorans)based on 16S rDNA sequence analysis. Pantoea ananatis was the most frequently isolated organism (70