Expression of Foreign Genes in Dunaliella by Electroporation 

 -  Molecular Biotechnology, 30, p.185-192, 2005 .
<br/><br/> An electroporation procedure has been described for introducing plasmid DNA into Dunaliella salina cells. By this procedure, a bulk of plasmid DNA was delivered into the cells and retained for at least 3 d. Reverse transcriptase polymerase chain reaction (RT-PCR)and sequencing analyses indicated that the transcription and pre-mRNA splicing of ble gene (contributing the Zeocin resistance)were detected in the cells as early as 1 h after the electroporation. Individual colonies could retain the resistance to 10 mg/L Zeocin for at least 6 mo. Subsequent Southern blot analysis showed the existence of introduced plasmid DNA inside these colonies. However, most of the cells (approx 90 
<br/><br/><label>Subjects--Topical Terms: </label><br/>DUNALIELLA SALINA<br/>ELECTROPORATION<br/>BLE GENE<br/>ZEOCIN<br/>EPISOMAL DNA