TY  - BOOK
AU  - Sun,Y.
AU  - Yang,Z.
AU  - Gao,X.
AU  - Li,Q.
AU  - Zhang,Q.
AU  - Xu,Z.
TI  - Expression of Foreign Genes in Dunaliella by Electroporation
KW  - DUNALIELLA SALINA
KW  - ELECTROPORATION
KW  - BLE GENE
KW  - ZEOCIN
KW  - EPISOMAL DNA
N2  - An electroporation procedure has been described for introducing plasmid DNA into Dunaliella salina cells. By this procedure, a bulk of plasmid DNA was delivered into the cells and retained for at least 3 d. Reverse transcriptase polymerase chain reaction (RT-PCR)and sequencing analyses indicated that the transcription and pre-mRNA splicing of ble gene (contributing the Zeocin resistance)were detected in the cells as early as 1 h after the electroporation. Individual colonies could retain the resistance to 10 mg/L Zeocin for at least 6 mo. Subsequent Southern blot analysis showed the existence of introduced plasmid DNA inside these colonies. However, most of the cells (approx 90
UR  - https://drive.google.com/file/d/1GZeWNNZwIMb_zvc9WZOwT2XYQEVBznbs/view?usp=drivesdk
ER  -