TY  - BOOK
AU  - Mitra,A.
AU  - Kitamura,Y.
AU  - Gasson,M.J.
AU  - Narbad,A.
AU  - Parr,A.J.
AU  - Payne,J.
AU  - Rhodes,M.J.C.
AU  - Rhodes,M.J.C.
AU  - Walton,N.J.
TI  - 4-Hydroxycinnamoyl-CoA Hydratase/lyase (HCHL)ÐAn Enzyme of Phenylpropanoid Chain Cleavage from Pseudomonas
KW  - PSEUDOMONAS FLUORESCENS
KW  - HYDROXYCINNAMIC ACID
KW  - PHENYLPROPANOID
KW  - FERULIC ACID
KW  - VANILLIN
KW  - ENOYL-COA HYDRATASE
N2  - The enzyme 4-hydroxycinnamoyl-CoA hydratase/ lyase (HCHL), which catalyzes a hydration and twocarbon cleavage step in the degradation of 4-hydroxycinnamic acids, has been purified and characterized from Pseudomonas fluorescens strain AN103. The enzyme is a homodimer and is active with three closely related substrates, 4-coumaroyl-CoA, caffeoyl-CoA, and feruloyl-CoA (Km values: 5.2, 1.6, and 2.4 mM, respectively), but not with cinnamoyl-CoA or with sinapinoyl- CoA. The abundance of the enzyme reflects a low catalytic center activity (2.3 molecules s21 at 30°C; 4-coumaroyl-CoA as substrate)
UR  - https://drive.google.com/file/d/1kFdlXXGItDxo2e7WJP0VFCFnh8vcSVsu/view?usp=drivesdk
ER  -