Enzymatic Synthesis and Purification of Aromatic Coenzyme A Esters 

 -  Analytical BioChemistry, 302(2), p.305-312, 2002 .
<br/><br/> Two recombinant His-tagged proteins, a plant 4-coumarate: coenzyme A ligase (EC 6.2.1.12)and a bacterial benzoate:coenzyme A ligase (EC 6.2.1.25), were expressed in Escherichia coli and purified in a single step using Ni-chelating chromatography. Purified enzymes were used to synthesize cinnamoyl-coenzyme A (CoA), p-coumaroyl-CoA, feruloyl-CoA, caffeoyl-CoA, and benzoyl-CoA. Conversions up to 95 
<br/><br/><label>Subjects--Topical Terms: </label><br/>COENZYME A<br/>ELECTROSPRAY IONIZATION-MASS SPECTROMETRY<br/>AFFINITY CHROMATOGRAPHY<br/>SOLIDPHASE EXTRACTION<br/>4-COUMARATE<br/>COA LIGASE<br/>BENZOATE<br/>COA LIGASE<br/>RECOMBINANT PROTEINS