TY  - BOOK
AU  - Beuerle,T.
AU  - Pichersky,E.
TI  - Enzymatic Synthesis and Purification of Aromatic Coenzyme A Esters
KW  - COENZYME A
KW  - ELECTROSPRAY IONIZATION-MASS SPECTROMETRY
KW  - AFFINITY CHROMATOGRAPHY
KW  - SOLIDPHASE EXTRACTION
KW  - 4-COUMARATE
KW  - COA LIGASE
KW  - BENZOATE
KW  - RECOMBINANT PROTEINS
N2  - Two recombinant His-tagged proteins, a plant 4-coumarate: coenzyme A ligase (EC 6.2.1.12)and a bacterial benzoate:coenzyme A ligase (EC 6.2.1.25), were expressed in Escherichia coli and purified in a single step using Ni-chelating chromatography. Purified enzymes were used to synthesize cinnamoyl-coenzyme A (CoA), p-coumaroyl-CoA, feruloyl-CoA, caffeoyl-CoA, and benzoyl-CoA. Conversions up to 95
UR  - https://drive.google.com/open?id=1xLxsFGbOdldkk0f1u5wYqNSORLCCrbmq&usp=drive_copy
ER  -