Somatic embryogenesis and plantlet production using rejuvenated tissues from serial grafting of a mature Kalopanax septemlobus tree - In Vitro Cellular & Developmental Biology-Plant, 44(2), p.119-127, 2008 .

An effective micropropagation technique via somatic embryogenesis has been developed using tissue from serially grafted shoots generated from a mature Kalopanax septemlobus tree (~40 y old). Callus was induced from leaf segments obtained from the grafts by culturing the explants in Murashige and Skoog (MS)medium supplemented with 2,4-D and 3


KALOPANAX SEPTEMLOBUS
SOMATIC EMBRYOGENESIS
REJUVENATION
GRAFTING
COCONUT WATER
POLYETHYLENE GLYCOL