A Simple Silica-based Method for Metagenomic DNA Extraction from Soil and Sediments 

 -  Molecular Biotechnology, 40(1), p.13-17, 2008 .
<br/><br/> A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. Highquality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples 
<br/><br/><label>Subjects--Topical Terms: </label><br/>METAGENOMIC DNA<br/>MICROBIAL COMMUNITY<br/>SEDIMENTS<br/>SOILS<br/>DGGE