TY  - BOOK
AU  - Rojas-Herrera,R.
AU  - Narváez-Zapata,J.
AU  - Zamudio-Maya,M.
AU  - Mena-Martínez,M.E.
TI  - A Simple Silica-based Method for Metagenomic DNA Extraction from Soil and Sediments
KW  - METAGENOMIC DNA
KW  - MICROBIAL COMMUNITY
KW  - SEDIMENTS
KW  - SOILS
KW  - DGGE
N2  - A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. Highquality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples
UR  - https://drive.google.com/file/d/1uGN7ygfoHLFfEf6Vcm_zY69fIX3Tt5Ex/view?usp=drivesdk
ER  -