Apolar chromatography on Sephadex LH-20 combined with high-speed counter-current chromatography High yield strategy for structurally closely related analytes-Destruxin derivatives from Metarhizium anisopliae as a case study 

 -  Journal of Chromatography A, 1117(1), p.67-73, 2006 .
<br/><br/> A novel high yield isolation procedure for lipophilic cyclic peptide derivatives is presented. Destruxin (dtx)A, B, D, E, and E-diol retrieval from Metarhizium anisopliae culture broth was achieved with a three-step purification protocol. After liquid-liquid extraction column chromatography over Sephadex LH-20 served as enrichment step. High-speed counter-current chromatography (HSCCC)was used for the final purification.Within the first chromatographic step dtx D and dtx E-diol were separated in purities exceeding 90 
<br/><br/><label>Subjects--Topical Terms: </label><br/>METARHIZIUM ANISOPLIAE<br/>DESTRUXINS<br/>SEPHADEX-LH20<br/>HSCCC<br/>HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY