TY  - BOOK
AU  - Seger,C.
AU  - Eberhart,K.
AU  - Sturm,S.
AU  - Strasser,H.
AU  - Stuppner,H.
TI  - Apolar chromatography on Sephadex LH-20 combined with high-speed counter-current chromatography High yield strategy for structurally closely related analytes-Destruxin derivatives from Metarhizium anisopliae as a case study
KW  - METARHIZIUM ANISOPLIAE
KW  - DESTRUXINS
KW  - SEPHADEX-LH20
KW  - HSCCC
KW  - HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY
N2  - A novel high yield isolation procedure for lipophilic cyclic peptide derivatives is presented. Destruxin (dtx)A, B, D, E, and E-diol retrieval from Metarhizium anisopliae culture broth was achieved with a three-step purification protocol. After liquid-liquid extraction column chromatography over Sephadex LH-20 served as enrichment step. High-speed counter-current chromatography (HSCCC)was used for the final purification.Within the first chromatographic step dtx D and dtx E-diol were separated in purities exceeding 90
UR  - https://drive.google.com/file/d/1kS_2wsFNxA-aSBntOAfZ2BlVnGmu5BbH/view?usp=drivesdk
ER  -