Expression of a Foreign Gene in Chlamydomonas reinhardtii Chloroplast 

 -  Journal of BioScience and BioEngineering, 87(3), p.307-314, 1999 .
<br/><br/> Chimeric genes for expression of a foreign gene in the Chlamydomonas reinhardtii chloroplast were constructed. These chimeric genes are composed of the promoter from chloroplast genes, rbcL, psbA, and atpA, S- and S/-untranslated regions, and the Escherichia coli ,9-glucuronidase (GUS)structural gene (uidA)as a foreign gene. Three types of chloroplast transformants (RG, PG, and AG), which contained the rbcL-uidA, psbA-uidA, and atpA-uidA chimerlc genes integrated in the chloroplast genome, were generated by particle bombardment. The AG transformant grown under photoautotrophlc conditions showed the highest GUS activity (130 nmol/min/mg protein)so far reported in C. reinhurdtii, and the accumulated GUS protein accounted for 0.08 
<br/><br/><label>Subjects--Topical Terms: </label><br/>CHLAMYDOMONAS REINHARDTII<br/>CHLOROPLAST<br/>TRANSFORMATION<br/>PROMOTER<br/>EXPRESSION<br/>P-GLUCURONIDASE