TY  - BOOK
AU  - Ishikura,K.
AU  - Takaoka,Y.
AU  - Kato,K.
AU  - Sekine,M.
AU  - Yoshida,K.
TI  - Expression of a Foreign Gene in Chlamydomonas reinhardtii Chloroplast
KW  - CHLAMYDOMONAS REINHARDTII
KW  - CHLOROPLAST
KW  - TRANSFORMATION
KW  - PROMOTER
KW  - EXPRESSION
KW  - P-GLUCURONIDASE
N2  - Chimeric genes for expression of a foreign gene in the Chlamydomonas reinhardtii chloroplast were constructed. These chimeric genes are composed of the promoter from chloroplast genes, rbcL, psbA, and atpA, S- and S/-untranslated regions, and the Escherichia coli ,9-glucuronidase (GUS)structural gene (uidA)as a foreign gene. Three types of chloroplast transformants (RG, PG, and AG), which contained the rbcL-uidA, psbA-uidA, and atpA-uidA chimerlc genes integrated in the chloroplast genome, were generated by particle bombardment. The AG transformant grown under photoautotrophlc conditions showed the highest GUS activity (130 nmol/min/mg protein)so far reported in C. reinhurdtii, and the accumulated GUS protein accounted for 0.08
UR  - https://drive.google.com/file/d/132OEbRdj6o33yG9wFEFXjOmbC5kSJtIs/view?usp=drivesdk
ER  -