TY  - BOOK
AU  - Lu,Y.
AU  - Luo,J.
AU  - Xu,D.
AU  - Huang,X.
AU  - Kong,L.
TI  - Characterization of spirostanol saponins in Solanum torvum by high-performance liquid chromatography/ evaporative light scattering detector/electrospray ionization with multi-stage tandem mass spectrometry
N2  - High-performance liquid chromatography with an evaporative light scattering detector and electrospray ionization multistage tandem mass spectrometry (HPLC/ELSD/ESI-MSn)was used to identify spirostanol saponins in a saponin extract of Solanum torvum. The fragmentation behavior of saponins was studied using ESI-MS1-3 in positive ion mode. The MSn spectra of the [MRH]R ions provide structural information including aglycone type and the nature and sequence of sugars. The use of ELSD allowed the profiling of the nonchromophore-containing saponins in this plant. The MS analysis established in this study with known saponins was successfully applied to tentatively identify two new siprostanol glycosides, neosolaspigenin 6-O-b-D-quinovopyranoside and solagenin 6-O-[b-D-xylopyranosyl-(1!3)-O-b-D-quinovopyranoside]
UR  - https://drive.google.com/file/d/1G5SOMufsx4Yz7jiHKxiZDonlT90c3TdH/view?usp=drivesdk
ER  -