Development and Validation of an HPLC Method for the Characterization and Assay of the Saponins from Ilex paraguariensis A. St.-Hil (Mate)Fruits 

 -  Journal of Liquid Chromatography & Related Technologies, 30(1), p.87-95, 2007 .
<br/><br/> Two improved HPLC methods for the characterization and assay of the saponin content in green fruits of Ilex paraguariensis (erva-mate)are described. Both HPLC methods consisted of isocratic separation at room temperature, using a RP-18 column with UV-Vis detection. The first system was intended for the characterization of the main I. paraguariensis saponin fraction, while the second one was specifically developed and validated for its quantitation. Matesaponin-3 was used as external standard. From the freeze dried extract, ten peaks were characterized as saponins by TLC and HPLC analysis. The matesaponin-3 content was 5.13 g 
<br/><br/><label>Subjects--Topical Terms: </label><br/>ILEX PARAGUARIENSIS<br/>FRUITS<br/>SAPONINS<br/>HPLC ASSAY<br/>VALIDATION