TY  - BOOK
AU  - Pavei,C.
AU  - Guzatto,P.
AU  - Ros Petrovick,P.
AU  - Gosmann,G.
AU  - González-Ortega,G.
TI  - Development and Validation of an HPLC Method for the Characterization and Assay of the Saponins from Ilex paraguariensis A. St.-Hil (Mate)Fruits
KW  - ILEX PARAGUARIENSIS
KW  - FRUITS
KW  - SAPONINS
KW  - HPLC ASSAY
KW  - VALIDATION
N2  - Two improved HPLC methods for the characterization and assay of the saponin content in green fruits of Ilex paraguariensis (erva-mate)are described. Both HPLC methods consisted of isocratic separation at room temperature, using a RP-18 column with UV-Vis detection. The first system was intended for the characterization of the main I. paraguariensis saponin fraction, while the second one was specifically developed and validated for its quantitation. Matesaponin-3 was used as external standard. From the freeze dried extract, ten peaks were characterized as saponins by TLC and HPLC analysis. The matesaponin-3 content was 5.13 g
UR  - https://drive.google.com/file/d/1mu6BQfGbt9BZyE0CHe3LlkhQOFi5dW5S/view?usp=drivesdk
ER  -