Application of acetate derivatives for gas chromatography-mass spectrometry: Novel approaches on carbohydrates, lipids and amino acids analysis 

 -  Journal of Chromatography A, 1208(1-2), p.215-222, 2008 .
<br/><br/> The structure of glycoconjugates has been determined by several chromatographic methods, however gas chromatography-mass spectrometry (GC-MS)has been widely used to identify and quantify the volatile trimethylsilyl and fluoroacyl derivatives. Adapting the reduction/acetylation strategies, we had performed the derivatization of all monosaccharide class, as well as amino acids and OH-fatty acids as fromdifferent glycoconjugates. Uronic acids gave characteristic ions atm/z 143, 156 and 173, and 19 amino acids derivatives, gave molecular ions [M]+ and daughter ions of [M-59]+ and [M-43]+ on electron impact (EI)-MS, which provide their rapid identification 
<br/><br/><label>Subjects--Topical Terms: </label><br/>ALDITOL-ACETATE-METHYL-ESTERS<br/>ACETATE-METHYL-ESTERS<br/>URONIC ACIDS<br/>AMINO ACIDS<br/>LIPIDS<br/>GC-MS