TY - BOOK AU - Bates,G.W. TI - Electroporation of protoplasts KW - DIRECT GENE TRANSFER KW - STABLE TRANSFORMATION KW - DNA UPTAKE KW - TRANSIENT EXPRESSION KW - CAT EXPRESSION KW - KANAMYCIN RESISTANCE N2 - A protocol is described for the delivery- of plasmid DNA into plant protoplasts by means of electroporation. Expression of the DNA is monitored 24 h later by a "transient assay" for chloramphenicol acetyltransferase (CATL Stable transformants are recovered by selecting for kanamycin resistance using a modified version of the agarose-bead technique UR - https://drive.google.com/file/d/1A_tKEEkhQO5C3gQCFaPwy9R3bITrF3_J/view?usp=drivesdk ER -