Effect of hemicellulose and lignin on enzymatic hydrolysis of cellulose from brewer's spent grain
- Enzyme and Microbial Technology, 43(2), p.124-129, 2008 .
Enzymatic hydrolysis of brewerfs spent grain in three different forms: original (untreated), pretreated by dilute acid (cellulignin), and pretreated by a sequence of dilute acid and dilute alkali (cellulose pulp), was studied to verify the effect of hemicellulose and lignin on cellulose conversion into glucose. The hydrolysis was carried out using a commercial cellulase concentrate (Celluclast 1.5 L)in an enzyme/substrate ratio of 45 FPU/g, 2