Interactions of N-ethylmaleimide and aluminium fluoride with GABA B receptor function in rat neocortical slices 

 -  European Journal of Pharmacology, 287(2), p.197-200, 1995 .
<br/><br/> Interactions of N-ethylmaleimide and aluminium fluoride (AlF 4)with GABA B receptors have been examined using spontaneously discharging rat neocortical slices. The suppression of discharges by the GABA B receptor agonist baclofen (5-10 /zM)was irreversibly prevented by N-ethylmaleimide (10-50 tzM)and its analog N-phenylmaleimide (10-50 /zM), whilst superfusion of slices with NaF (10 mM)and A1C13 (100 ~M)to form a fluoroaluminate (AIF4-)complex markedly potentiated the action of baclofen. The lipoxygenase inhibitors, nordihydroguaiaretic acid (10-50/~M)and eicosatetraynoic acid (10-50/~M)or the phospholipase A 2 inhibitor bromophenacylbromide (50-100/~M)did not affect the response to baclofen. The depressant action of baclofen is evidently mediated through G-proteins, but is not dependent on arachidonic acid metabolites 
<br/><br/><label>Subjects--Topical Terms: </label><br/>GABA B RECEPTOR<br/>BACLOFEN<br/>NEOCORTICAL SLICE<br/>RAT<br/>N-ETHYLMALEIMIDE<br/>ALUMINUM FLUORIDE<br/>G-PROTEIN