TY  - BOOK
AU  - Vigneault,F.
AU  - Michael Sismour,A.
AU  - Church,G.M.
TI  - Efficient microRNA capture and bar-coding via enzymatic oligonucleotide adenylation
N2  - Here we report a highly efficient and simplified strategy to preadenylate bar-coded oligonucleotides designed for microRNA (miRNA)capture and multiplex analysis. Using this approach, we enzymatically preadenylated bar-coded oligonucleotides with high efficiency when compared to the chemical method currently used by miRNA investigators. As a case study, we used these oligonucleotides in an ATP-independent ligation to miRNAs, suggesting the utility of our method in end-capture protocols and high-throughput sequencing applications
UR  - https://drive.google.com/file/d/14KG349UEHYc5WFS7l2_wCD6l6WwoAMZv/view?usp=drivesdk
ER  -