TY  - BOOK
AU  - Shachar,S.
AU  - Ziv,O.
AU  - Avkin,S.
AU  - Adar,S.
AU  - Wittschieben,J.
AU  - Reißner,T.
AU  - Chaney,S.
AU  - Chaney,S.
AU  - Wang,Z.
AU  - Carell,T.
AU  - Geacintov,N.
AU  - Livneh,Z.
TI  - Two-polymerase mechanisms dictate error-free and error-prone translesion DNA synthesis in mammals
N2  - DNA replication across blocking lesions occurs by translesion DNA synthesis (TLS), involving a multitude of mutagenic DNA polymerases that operate to protect the mammalian genome. Using a quantitative TLS assay, we identified three main classes of TLS in human cells: two rapid and error-free, and the third slow and error-prone. A single gene, REV3L, encoding the catalytic subunit of DNA polymerase f (polf), was found to have a pivotal role in TLS, being involved in TLS across all lesions examined, except for a TT cyclobutane dimer. Genetic epistasis siRNA analysis indicated that discrete two-polymerase combinations with polf dictate error-prone or error-free TLS across the same lesion. These results highlight the central role of polf in both error-prone and error-free TLS in mammalian cells, and show that bypass of a single lesion may involve at least three different DNA polymerases, operating in different two-polymerase combinations
UR  - https://drive.google.com/file/d/1Wqf0FvzoMe0XzMNY2ptdW7qTgUVU-8A2/view?usp=drivesdk
ER  -