TY  - BOOK
AU  - Kim,S.T.
AU  - Kang,Y.H.
AU  - Wang,Y.
AU  - Wu,J.
AU  - Park,Z.Y.
AU  - Rakwal,R.
AU  - Agrawal,G.K.
AU  - Agrawal,G.K.
AU  - Kang,K.Y.
TI  - Secretome analysis of differentially induced proteins in rice suspension-cultured cells triggered by rice blast fungus and elicitor
KW  - ELICITOR
KW  - MALDI-TOF-MS
KW  - M. GRISEA
KW  - SECRETOME
KW  - SUSPENSION-CULTURED CELL
N2  - Secreted proteins were investigated in rice suspension-cultured cells treated with rice blast fungus Magnaporthe grisea and its elicitor using biochemical and 2-DE coupled with MSanalyses followed by their in planta mRNA expression analysis. M. grisea and elicitor successfully interacted with suspension-cultured cells and prepared secreted proteins from these cultures were essentially intracellular proteins free. Comparative 2-D gel analyses identified 21 differential protein spots due to M. grisea and/or elicitor over control. MALDI-TOF-MS and mLC-ESI-MS/MS analyses of these protein spots revealed that most of assigned proteins were involved in defense such as nine chitinases, two germin A/oxalate oxidases, five domain unknown function 26 (DUF 26)secretory proteins, and b-expansin. One chitin binding chitinase protein was isolated using chitin binding beads and strong enzymatic activity was identified in an in-gel assay. Interestingly, their protein abundance correlated well at transcript levels in elicitor-treated cultures as judged by semi-quantitative RT-PCR. Each identified differentially expressed protein group was compared at transcript levels in rice leaves inoculated with incompatible (KJ401)and compatible (KJ301)races of M. grisea. Time-course profiling revealed their inductions were stronger and earlier in incompatible than compatible interactions. Identified secreted proteins and their expression correlation at transcript level in suspension-cultured cells and also in planta suggest that suspension-cultured cells can be useful to investigate the secretome of rice blast-pathogen interactions
UR  - https://drive.google.com/file/d/14edaSBmB-Ww6pGgqwzAK_g_chvXDRAdw/view?usp=drivesdk
ER  -