TY - BOOK AU - Joh,L.D. AU - Wroblewski,T. AU - Ewing,N.N. AU - Van Der Gheyns,J.S. TI - High-Level Transient Expression of Recombinant Protein in Lettuce KW - AGROINFILTRATION KW - LETTUCE KW - AGROBACTERIUM KW - BGLUCURONIDASE KW - TRANSIENT EXPRESSION KW - RECOMBINANT PROTEIN PRODUCTION N2 - Transient expression following agroinfiltration of plant tissue was investigated as a system for producing recombinant protein. As a model system, Agrobacterium tumefaciens containing the b-glucuronidase (GUS)gene was vacuum infiltrated into lettuce leaf disks. Infiltration with a suspension of 109 colony forming units/mL followed by incubation for 72 h at 228C in continuous darkness produced a maximum of 0.16 UR - https://drive.google.com/file/d/1F-ICSrWfwCUG-xjGfzuz-5VN1k70LbPS/view?usp=drivesdk ER -