Towards crop improvement in bell pepper (Capsicum annuum L.): Transgenics (uid A::hpt II)by a tissue-culture-independent grobacterium-mediated in planta approach 

 -  Scientia Horticulturae, 119(4), p.362-370, 2009 .
<br/><br/> Agrobacterium tumefaciens-mediated transformation has been one of the methods used to generate transgenic plants in bell pepper. An alternate transformation method that avoids/minimizes tissue culture would be beneficial for the improvement of bell pepper due to its recalcitrant nature. In this report, transgenic bell pepper plants have been developed by a tissue-culture-independent A. tumefaciens-mediated in planta transformation procedure. In the present study, two open pollinated varieties viz., Arka Gaurav and Arka Mohini were used for transformation. Agrobacterium train EHA105 harboring the binary vector pCAMBIA1301 that carries the genes for b-glucuronidase (uid A)and hygromycin phosphotransferase II (hpt II)was used for transformation. GUS histochemical analysis of T0 and T1 plants at various stages of growth followed by molecular analysis using PCR, Southern analysis and RT-PCR allowed selection of transgenics. The method resulted in 17.8 
<br/><br/><label>Subjects--Topical Terms: </label><br/>CAPSICUM ANNUUM<br/>BELL PEPPER<br/>IN PLANTA TRANSFORMATION<br/>AGROBACTERIUM<br/>MARKER GENES<br/>TRANSGENICS