Recombination and expression of classical swine fever virus (CSFV)structural protein E2 gene in Chlamydomonas reinhardtii chroloplasts 

 -  Colloids and Surfaces B: Biointerfaces, 55(1), p.26-30, 2007 .
<br/><br/> The expression of classical swine fever virus (CSFV)structural protein E2 in different vectors, which has been shown to carry critical epitopes, has been established. Here, we reported a Chlamydomonas reinhardtii chloroplast expression vector, P64E2, containing classical swine fever virus structural protein E2 gene, which was constructed and transferred to C. reinhardtii by biolistic bombardment method. The transformants were identified by PCR, Southern blotting, Western blotting after selecting on resistant media. ELISA quantification assay showed that the expressed E2 protein accumulated up to 1.5-2 
<br/><br/><label>Subjects--Topical Terms: </label><br/>CLASSICAL SWINE FEVER VIRUS (CSFV)<br/>E2 GENE<br/>CHLAMYDOMONAS REINHARDTII<br/>CHLOROPLAST TRANSFORMATION<br/>ANTI-DISEASE PROTEIN<br/>BIOINTERFACE<br/>PLANT GENE ENGINEERING