TY  - BOOK
AU  - Krijger,J.J.
AU  - Horbach,R.
AU  - Behr,M.
AU  - Schweizer,P.
AU  - Deising,H.B.
AU  - Wirsel,S.G.R.
TI  - The Yeast Signal Sequence Trap Identifies Secreted Proteins of the Hemibiotrophic Corn Pathogen Colletotrichum graminicola
KW  - BIOTROPHY
KW  - CELL-WALL-DEGRADING ENZYMES
KW  - EFFECTOR PROTEINS
KW  - NECROTROPHY
KW  - PEPTIDASES
N2  - The hemibiotroph Colletotrichum graminicola is the causal agent of stem rot and leaf anthracnose on Zea mays. Following penetration of epidermal cells, the fungus enters a short biotrophic phase, followed by a destructive necrotrophic phase of pathogenesis. During both phases, secreted fungal proteins are supposed to determine progress and success of the infection. To identify genes encoding such proteins, we constructed a yeast signal sequence trap (YSST)cDNA-library from RNA extracted from mycelium grown in vitro on corn cell walls and leaf extract. Of the 103 identified unigenes, 50 showed significant similarities to genes with a reported function, 25 sequences were similar to genes without a known function, and 28 sequences showed no similarity to entries in the databases. Macroarray hybridization and quantitative reverse-transcriptase polymerase chain reaction confirmed that most genes identified by the YSST screen are expressed in planta. Other than some genes that were constantly expressed, a larger set showed peaks of transcript abundances at specific phases of pathogenesis. Another set exhibited biphasic expression with peaks at the biotrophic and necrotrophic phase. Transcript analyses of in vitro-grown cultures revealed that several of the genes identified by the YSST screen were induced by the addition of corn leaf components, indicating that host-derived factors may have mimicked the host milieu
UR  - https://drive.google.com/file/d/1vOJopYinbvvG-k26bMdLjc9seIdDSI2R/view?usp=drivesdk
ER  -