Transposon-tagging identifies novel pathogenicity genes in Fusarium graminearum 

 -  Fungal Genetics and Biology, 45(12), p.1552-1561, 2008 .
<br/><br/> With the increase of sequenced fungal genomes, high-throughput methods for functional analyses of genes are needed. We assessed the potential of a new transposon mutagenesis tool deploying a Fusarium oxysporum miniature inverted-repeat transposable element mimp1, mobilized by the transposase of impala, a Tc1-like transposon, to obtain knock-out mutants in Fusarium graminearum. We localized 91 mimp1 insertions which showed good distribution over the entire genome. The main exception was a major hotspot on chromosome 2 where independent insertions occurred at exactly the same nucleotide position. Furthermore insertions in promoter regions were over-represented. Screening 331 mutants for sexual development, radial growth and pathogenicity on wheat resulted in 19 mutants (5.7 
<br/><br/><label>Subjects--Topical Terms: </label><br/>TRANSPOSON MUTAGENESIS<br/>FUSARIUM GRAMINEARUM<br/>FUNCTIONAL SCREENING<br/>PATHOGENICITY<br/>SEXUAL DEVELOPMENT