TY  - BOOK
AU  - Dufresne,M.
AU  - Van Der Lee,T.
AU  - M'Barek,S.B.
AU  - Xu,X.
AU  - Zhang,X.
AU  - Liu,T.
AU  - Waalwijk,C.
AU  - Waalwijk,C.
AU  - Kema,G.H.J.
AU  - Daboussi,M.J.
TI  - Transposon-tagging identifies novel pathogenicity genes in Fusarium graminearum
KW  - TRANSPOSON MUTAGENESIS
KW  - FUSARIUM GRAMINEARUM
KW  - FUNCTIONAL SCREENING
KW  - PATHOGENICITY
KW  - SEXUAL DEVELOPMENT
N2  - With the increase of sequenced fungal genomes, high-throughput methods for functional analyses of genes are needed. We assessed the potential of a new transposon mutagenesis tool deploying a Fusarium oxysporum miniature inverted-repeat transposable element mimp1, mobilized by the transposase of impala, a Tc1-like transposon, to obtain knock-out mutants in Fusarium graminearum. We localized 91 mimp1 insertions which showed good distribution over the entire genome. The main exception was a major hotspot on chromosome 2 where independent insertions occurred at exactly the same nucleotide position. Furthermore insertions in promoter regions were over-represented. Screening 331 mutants for sexual development, radial growth and pathogenicity on wheat resulted in 19 mutants (5.7
UR  - https://drive.google.com/file/d/1MSS-w76mEjoj3obUnV2bjNAbd50Kg1bL/view?usp=drivesdk
ER  -