TY  - BOOK
AU  - An,Z.W.
AU  - Xie,L.L.
AU  - Cheng,H.
AU  - Zhou,Y.
AU  - Zhang,Q.
AU  - He,X.G.
AU  - Huang,H.S.
TI  - A silver staining procedure for nucleic acids in polyacrylamide gels without fixation and pretreatment
N2  - Silver staining of nucleic acid has been widely used in molecular marker analysis such as simple sequence repeat (SSR), single-strand conformation polymorphism (SSCP), and amplified fragment-length polymorphism (AFLP). Many alternatives to silver staining methods have been described, but these methods are not efficient or cost-effective. Here we report a silver staining method that requires less than 10 min for one gel and can save chemicals as well. It has a detection limit of approximately 5.6 pg of DNA/mm2 in nondenaturing polyacrylamide gels and 12.8 pg/mm2 in denaturing polyacrylamide gels
UR  - https://drive.google.com/file/d/1ikCCnAkDuaZ9BTzSkWlHJurbYiowYmdd/view?usp=drivesdk
ER  -