An improved HPLC assay for phosphatidylcholine hydroperoxides (PCOOH)in human plasma with synthetic PCOOH as internal standard 

 -  Journal of Chromatography B, 857(1), p.158-163, 2007 .
<br/><br/> Quantitative and qualitative analyses of 1-palmitoyl-2-linoleoyl-phosphatidylcholine monohydroperoxide [PC 16:0/18:2-OOH]and 1-stearoyl-2-linoleoyl-phosphatidylcholine monohydroperoxide [PC 18:0/18:2-OOH]in human plasma were improved by chemiluminescence HPLC usingsynthetic 1-stearoyl-2-erucoyl-phosphatidylcholine monohydroperoxide (PC 18:0/22:1-OOH)as internal standard. The calibration curves of synthetic PC 16:0/18:2-OOH and PC 18:0/18:2-OOH, obtained by their direct injections with the IS into the HPLC system, were linear throughout the calibration range (10-1000 pmol).Within-day and between-day coefficients of variation were below 
<br/><br/><label>Subjects--Topical Terms: </label><br/>HPLC<br/>CHEMILUMINESCENCE<br/>LUMINOL<br/>PHOSPHATIDYLCHOLINE HYDROPEROXIDE<br/>PC<br/>HUMAN PLASMA