TY - BOOK AU - Hui,S. AU - Chiba,H. AU - Sakurai,T. AU - Asakawa,C. AU - Nagasaka,H. AU - Murai,T. AU - Ide,H. AU - Ide,H. TI - An improved HPLC assay for phosphatidylcholine hydroperoxides (PCOOH)in human plasma with synthetic PCOOH as internal standard KW - HPLC KW - CHEMILUMINESCENCE KW - LUMINOL KW - PHOSPHATIDYLCHOLINE HYDROPEROXIDE KW - PC KW - HUMAN PLASMA N2 - Quantitative and qualitative analyses of 1-palmitoyl-2-linoleoyl-phosphatidylcholine monohydroperoxide [PC 16:0/18:2-OOH]and 1-stearoyl-2-linoleoyl-phosphatidylcholine monohydroperoxide [PC 18:0/18:2-OOH]in human plasma were improved by chemiluminescence HPLC usingsynthetic 1-stearoyl-2-erucoyl-phosphatidylcholine monohydroperoxide (PC 18:0/22:1-OOH)as internal standard. The calibration curves of synthetic PC 16:0/18:2-OOH and PC 18:0/18:2-OOH, obtained by their direct injections with the IS into the HPLC system, were linear throughout the calibration range (10-1000 pmol).Within-day and between-day coefficients of variation were below UR - https://drive.google.com/file/d/1rWJifH9szRvCBIlhdgr9e2CZ4DbVb7Xv/view?usp=drivesdk ER -