Molecular characterization and identification of markers for toxic and non-toxic varieties of Jatropha curcas L. using RAPD, AFLP and SSR markers 

 -  Mol Biol Rep, 36(6), p.1357-1364, 2009 .
<br/><br/> Jatropha curcas L., a multipurpose shrub has acquired significant economic importance for its seed oil which can be converted to biodiesel, is emerging as an alternative to petro-diesel. The deoiled seed cake remains after oil extraction is toxic and cannot be used as a feed despite having best nutritional contents. No quantitative and qualitative differences were observed between toxic and non-toxic varieties of J. curcas except for phorbol esters content. Development of molecular marker will enable to differentiate non-toxic from toxic variety in a mixed population and also help in improvement of the species through marker assisted breeding programs. The present investigation was undertaken to characterize the toxic and non-toxic varieties at molecular level and to develop PCR based molecular markers for distinguishing non-toxic from toxic or vice versa. The polymorphic markers were successfully identified specific to non-toxic and toxic variety using RAPD and AFLP echniques. Totally 371 RAPD, 1,442 AFLP markers were analyzed and 56 (15.09 
<br/><br/><label>Subjects--Topical Terms: </label><br/>JATROPHA CURCAS L.<br/>NON-TOXIC<br/>TOXIC<br/>RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA)<br/>AFLP (AMPLIFIED FRAGMENT LENGTH POLYMORPHISM)<br/>MICROSATELLITES