Cryopreservation of embryogenic cell suspensions of Catharanthus roseus L. (G)Don. 

 -  Plant Cell, Tissue and Organ Culture , 98, p.1-9, 2009 .
<br/><br/> An efficient cryopreservation protocol was established for embryogenic cell suspension cultures of Catharanthus roseus. This involved a vitrification-based cryopreservation method wherein embryogenic cells were exposed to a preculture/pretreatment medium prior to their immersion in liquid nitrogen. These cell suspension cultures were first initiated from friable embryogenic callus derived from hypocotyls of C. roseus on a medium containing 4.52 lM 2,4-Dichlorophenoxy acetic acid (2, 4-D). Among different sucrose (0.09-0.6 M)and sorbitol (0.2-0.6 M)levels evaluated during preculture, 0.4 M sucrose promoted highest cellular regrowth. Whereas, among pretreatments Dimethyl sulphoxide (DMSO)(5 or 10 
<br/><br/><label>Subjects--Topical Terms: </label><br/>CRYOPRESERVATION<br/>EMBRYOGENIC SUSPENSIONS<br/>VITRIFICATION SOLUTION<br/>PRE CULTURE<br/>PRE TREATMENT