TY  - BOOK
AU  - Leelavathi,S.
AU  - Reddy,V.S.
TI  - Chloroplast expression of His-tagged GUS-fusions: a general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactors
KW  - AFFINITY CHROMATOGRAPHY
KW  - CHLOROPLAST EXPRESSION
KW  - GUS-FUSION
KW  - HIS-TAG
KW  - INTERFERON GAMMA
N2  - High level expression and efficient recovery of recombinant protein are two main critical factors that determine the use of transgenic plants as natural bioreactors to produce foreign proteins for industrial applications. We demonstrate here the potential of a new strategy involving chloroplast transformation, GUS-fusions and affinitytag based chromatography to overexpress and purify a human therapeutic protein, interferon gamma (IFN-g)in tobacco plants. Our results show that IFN-g accumulation reaches up to 6
UR  - https://drive.google.com/file/d/1Jceaelly_0w7PCDzOuV4-brym8gC7e2_/view?usp=drivesdk
ER  -