Manipulation of Microalgal Lipid Production Using Genetic Engineering 

 -  Applied BioChemistry and Biotechnology, 57/58, p.223-231, 1996 .
<br/><br/> Genetic transformation of two species of diatoms has been accomplished by introducing chimeric plasmid vectors containing a bacterial antibiotic resistance gene driven by regulatory sequences from the acetyl-CoA carboxylase (ACCase)gene from the diatom Cyclotella cryptica. The recombinant DNA integrated into one or more random sites within the algal genome and the foreign protein was pro- duced by the algal transformants. This is the first report of genetic transformation of any chlorophyll c-containing microalgal strain. We are using this system to introduce additional copies of the ACCase gene into diatoms in an attempt to mani- pulate lipid accumulation in transformed strains. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>BIODIESEL<br/>DIATOMS<br/>GENETIC TRANSFORMATION<br/>LIPID SYNTHESIS<br/>MICROALGAE