TY - BOOK AU - Dunahay,T.G. AU - Jarvls,E.E. AU - Dais,S.S. AU - Roessler,P.G. TI - Manipulation of Microalgal Lipid Production Using Genetic Engineering KW - BIODIESEL KW - DIATOMS KW - GENETIC TRANSFORMATION KW - LIPID SYNTHESIS KW - MICROALGAE N2 - Genetic transformation of two species of diatoms has been accomplished by introducing chimeric plasmid vectors containing a bacterial antibiotic resistance gene driven by regulatory sequences from the acetyl-CoA carboxylase (ACCase)gene from the diatom Cyclotella cryptica. The recombinant DNA integrated into one or more random sites within the algal genome and the foreign protein was pro- duced by the algal transformants. This is the first report of genetic transformation of any chlorophyll c-containing microalgal strain. We are using this system to introduce additional copies of the ACCase gene into diatoms in an attempt to mani- pulate lipid accumulation in transformed strains UR - https://drive.google.com/file/d/19y_KjnfG-NytRkeUov_NW7vi3QHEbUMr/view?usp=drivesdk ER -